Transformation of encapsulated Streptococcus pneumoniae.
نویسندگان
چکیده
We describe the high-efficiency transformation of several virulent, encapsulated isolates of Streptococcus pneumoniae. Transformation was effected by the induction of competence with competence factor and was apparently the result both of inducing noncompetent recipients and overcoming the inhibition imposed by the capsule.
منابع مشابه
Effect of intrastrain variation in the amount of capsular polysaccharide on genetic transformation of Streptococcus pneumoniae: implications for virulence studies of encapsulated strains.
The degrees of competence of opaque and transparent colony variants of Streptococcus pneumoniae were compared. The transparent variants were transformed at 9- to 670-fold-higher rates than the opaque variants, independent of the DNA incorporated, due to decreased expression of capsular polysaccharide. Genetic transformation, therefore, tends to select for a less-encapsulated subpopulation.
متن کاملNon-encapsulated Streptococcus pneumoniae, vaccination as a measure to interfere with horizontal gene transfer
Non-encapsulated Streptococcus pneumoniae, vaccination as a measure to interfere with horizontal gene transfer J.D. Langereis & M.I. de Jonge To cite this article: J.D. Langereis & M.I. de Jonge (2017): Non-encapsulated Streptococcus pneumoniae, vaccination as a measure to interfere with horizontal gene transfer, Virulence, DOI: 10.1080/21505594.2017.1309492 To link to this article: http://dx.d...
متن کاملCompetence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone.
The nucleotide sequence of comC, the gene encoding the 17-residue competence-stimulating peptide (CSP) of Streptococcus pneumoniae (L. S. Havarstein, G. Coomaraswamy, and D. A. Morrison, Proc. Natl. Acad. Sci. USA 92:11140-11144, 1995) was determined with 42 encapsulated strains of different serotypes. A new allele, comC2, was found in 13 strains, including the type 3 Avery strain, A66, while a...
متن کاملAdhesion and invasion of Streptococcus pneumoniae to primary and secondary respiratory epithelial cells
The interaction between Streptococcus pneumoniae (S. pneumoniae) and the mucosal epithelial cells of its host is a prerequisite for pneumococcal disease development, yet the specificity of this interaction between different respiratory cells is not fully understood. In the present study, three areas were examined: i) The capability of the encapsulated S. pneumoniae serotype 3 strain (WU2) to ad...
متن کاملType 3-specific synthase of Streptococcus pneumoniae (Cap3B) directs type 3 polysaccharide biosynthesis in Escherichia coli and in pneumococcal strains of different serotypes
The cap3B gene, which is involved in the formation of the capsule of Streptococcus pneumoniae type 3, encodes a 49-kD protein that has been identified as a polysaccharide synthase. Escherichia coli cells harboring the recombinant plasmid pTBP3 (cap3B) produced pneumococcal type 3 polysaccharide, as demonstrated by immunological tests. Biochemical and cell fractionation analyses revealed that th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 168 3 شماره
صفحات -
تاریخ انتشار 1986